Análisis de la capacidad antitumoral del té verde enriquecido con brócoli

Broccoli has risen as rich in bioactive phytochemicals (glucosinolates and phenolic compounds) closely linked with the reduction of cancer risk. Green tea infusion is a beverage that also contains anticarcinogenic compounds, mainly represented by flavanols. The compounds present in new broccoli-enriched green tea drinks and their potential antitumoral activity in vitro were evaluated. The distinct compounds present in the prepared beverages were identified by HPLC-PAD-ESI-MSn and quantified by HPLC-PAD. Caco-2 and CCD-18Co cell lines were exposed to growing percentages (0.2-5%) of infusions of distinct combinations of plant material. The time-dependent cytotoxicity on the malignant cells was also achieved. Cell death was evaluated by trypan blue dye exclusion and a more efficient specific cytotoxic effect on Caco-2 cells was observed on the cells incubated with the mixture of broccoli and green tea than on cells exposed to control infusions. Broccoli added to green tea resulted in a combination of phytochemicals with antitumoral activity with potential for further developments in mechanistic models and the design of novel foods.

Autores : Dominguez-Perles, R.; Moreno, D.A.; Garcia-Viguera, C.

Direcciones :

Departamento de Ciencias de la Alimentación y Tecnología, CEBAS, CSIC, Murcia (España)